Highly efficient oligonucleotide transfer into intact yeast cells using square-wave pulse electroporation.
نویسندگان
چکیده
Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.
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ورودعنوان ژورنال:
- BioTechniques
دوره 25 2 شماره
صفحات -
تاریخ انتشار 1998